Journal: Investigative Ophthalmology & Visual Science
Article Title: Transcriptomic and Immunohistochemical Analysis of Progressive Keratoconus Reveal Altered WNT10A in Epithelium and Bowman's Layer
doi: 10.1167/iovs.62.6.16
Figure Lengend Snippet: WNT10A overexpression in hTCEpi cells. Both WNT10A transcript ( A ) and protein levels ( C , D ) are elevated in the WNT10A overexpression plasmid wells (band size for WNT10A, 46 kDA; COL1A1, ∼120 kDA). COL1A1 transcript ( B ) and protein ( C , D ) are also increased in wells overexpressing WNT10A.
Article Snippet: The membrane was then suspended in a blocking buffer with the appropriate primary antibodies (either 1:1000 diluted primary anti-WNT10A rabbit polyclonal antibody [GTX111191; GeneTex, Irvine, CA, USA] or 1:2000 diluted primary anti-COL1A1 polyclonal sheep antibody [AF6220; R&D Systems, Minneapolis, MN, USA]) and incubated at 4°C overnight.
Techniques: Over Expression, Plasmid Preparation